Atherosclerosis. Altered colonic bacterial fermentation as a potential pathophysiological factor in irritable bowel syndrome. View more. Here, in vivo, changes in the metabolites in fecal samples by the mixture of probiotics that included Lactobacillus and Bifidobacterium were observed. Digestive & Gut Health - Supports Occasional Constipation, Diarrhea, Gas & Bloating - Probiotics For Women & Men. All Rights Reserved. To amplify the V3 and V4 regions, sequencing libraries were prepared according to the illumine 16S Metagenomic Sequencing Library protocols. Increasing levels of Akkermansia might significantly contribute to the increase in Verrucomicrobiota abundance identified by microbiome analysis here. Therefore, changes in the microbiome after treatment with microorganisms could be deeply related to changes in SCFAs. Bustle reached out to certified. Taken together, the multi-strain probiotics used in this experiment have the effect of improving constipation by secreting serotonin and mucin and have anti-inflammatory potential. A significant increase in SCFAs was observed, which are known to have positive effects on various diseases (Figure 4B). Metabolites were clearly distinguished in partial squares discriminant analysis depending on the increasing concentration of probiotics (Figure 5A). Probiotics are live, beneficial bacteria naturally found in fermented foods, including kombucha, kefir, sauerkraut, and tempeh. BMC Complement. A., and Holmes, S. P. (2016). The inaccessibility of the human small intestine, where probiotic action is occurring, makes studying interactions there difficult. Fermented foods like kimchi, kombucha, kefir, natto, tempeh, and sauerkraut are all rich in beneficial bacteria, as well as plenty of other important nutrients. The animal study was reviewed and approved by Animal Experimental Ethics Committee (IACUC) of Hallym University (Hallym 2021-79). (2014). Short-chain fatty acids stimulate colonic transit via intraluminal 5-HT release in rats. Acetic acid and butyric acid released in large intestine play different roles in the alleviation of constipation. doi: 10.1016/j.immuni.2013.12.007, Soret, R., Chevalier, J., De Coppet, P., Poupeau, G., Derkinderen, P., Segain, J. P., et al. (2022). 30, 772780. Front. On the other hand, the serotonin concentration in the G1 groups was 2.5ng/mL, that of G2 was 4.1ng/mL, and that of G3 was 4.0ng/mL, and the low-concentration G1 group did not show significant changes; however, it showed a slight increase (Figure 3). Weight. Research shows 5 g/d of PHGG to provide particular benefit to those with constipation, demonstrating the capacity to soften the stool, increase the weekly number of bowel motions, and decrease abdominal pain, straining with bowel motions, and laxative use. Claire is a qualified Naturopath holding eight years experience within the natural health industry, spanning both retail and clinical practice. Clin. *Correspondence: Byung-Yong Kim, bykim@ckdhc.com; Ki Tae Suk, ktsuk@hallym.ac.kr, These authors have contributed equally to this work, Industrial and Health Applications of Lactic Acid Bacteria and Their Metabolites, Volume II, View all Some foods can help relieve or reduce the risk of constipation, while others can make it worse. 17, 1012. Certain probiotics have demonstrated significant benefit for maintaining the health of the microbial community in the gut. Various causes of constipation are known, but it can result from intestinal obstruction, lack of exercise, low fiber intake, or personal factors (Bosaeus, 2004). Gastroenterology 126, 16571664. Enhanced production of IL-18 in butyrate-treated intestinal epithelium by stimulation of the proximal promoter region. Secretion of serotonin can be stimulated in the intestinal tract, and serotonin relay peristalsis of the intestinal tract occurs (Park, 2011). In another study in 20 women, taking probiotics containing a mix of bacteria strains increased bowel movement frequency and improved constipation symptoms like straining, stomach pain, and the sense of incomplete evacuation (14). of cDNA, 10pg. The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. Biochem. In addition, SCFAs can stimulate the secretion of serotonin and mucin, which can improve constipation (Hatayama et al., 2007; Reigstad et al., 2015). At Metagenics, transparency is one of our core values, so we make sure to list every . All authors contributed to manuscript revision, read, and approved the submitted version. On the other hand, in the group consuming butylated starch, constipation was alleviated by butyrate-producing bacteria, which had a negative correlation with the first black stool defecation time (Wang et al., 2020). doi: 10.1016/j.immuni.2013.08.003, Zhai, R., Xue, X., Zhang, L., Yang, X., Zhao, L., and Zhang, C. (2019). Di Cerbo A, Palmieri B, Aponte M, Morales-Medina JC, Iannitti T. Mechanisms and therapeutic effectiveness of lactobacilli. UltraFlora Spectrum. PMID: 9706795. 105, 48334841. Effect of dietary fibre on stools and transit-times, and its role in the causation of disease. To induce constipation, 5mg/kg loperamide was intraperitoneally administered twice a day for 7days to all groups except the normal control group. A multi-strain probiotics Consti-Biome containing a mixture of SynBalance SmilinGut (Lactobacillus plantarum PBS067, Lactobacillus rhamnosus LRH020, Bifidobacterium animalis subsp. Content of short-chain fatty acids in the hindgut of rats fed processed bean (Phaseolus vulgaris) flours varying in distribution and content of indigestible carbohydrates. UltraFlora Balance (formally Ultra Flora Plus DF Capsules) is a probiotic formula blended with a non-dairy formula that provides highly viable, pure strains of Lactobacillus acidophilus (NCFM strain) and Bifidobacterium lactis. Cytokines are important mediators of inflammation and immune responses and include Tnf-, Tnf-, Il-8, and Il-10. These cookies ensure basic functionalities and security features of the website, anonymously. (A) left indicate Principal Component Analysis (PCA) and right indicate Partial Least Squares Discriminant Analysis (PLS-DA) of metabolite profiling in fecal of loperamide administration (LOP), low concentration multi-strain probiotics administration (G1, 2108cfu/mL), medium concentration multi-strain probiotics administration (G2, 2109cfu/mL), and high concentration probiotic administration (G3, 21010cfu/mL) groups. doi: 10.1016/j.aca.2014.11.015, Hasani, A., Ebrahimzadeh, S., Hemmati, F., Khabbaz, A., Hasani, A., and Gholizadeh, P. (2021). (2017). Probiotic for Beneficial Gut Flora, Bowel Function and Immune Health in Children. Food Sci. Int J Food Microbiol. In both groups, the number of fecal was increased about 1.3 times compared to LOP. doi: 10.1007/s10350-006-0763-3, Dimidi, E., Scott, S. M., and Whelan, K. (2020). 10, S49S66. Bifidobacterium adolescentis exerts strain-specific effects on constipation induced by loperamide in BALB/c mice. Functional cookies help to perform certain functionalities like sharing the content of the website on social media platforms, collect feedbacks, and other third-party features. [6] Seddik HA, Bendali F, Gancel F, Fliss I, Spano G, Drider D. Lactobacillus plantarum and its probiotic and food potentialities. Figure 2. mRNA expression related to serotonin, cytokine, and mucin according to constipation induction and multi-strain probiotics Consti-Biome treatment. (2015). Body weight and food intake of the experimental animals were measured at weekly intervals. Constipation is common, but certain foods can provide relief. Pharmacother. doi: 10.18502/ijm.v11i5.1957, Kwoji, I. D., Aiyegoro, O. The role of Akkermansia muciniphila in obesity, diabetes and atherosclerosis. Pharm. Picking the right probiotic is key to treating constipation, as certain strains may not be as effective as others. In this study, the effect on loperamide-induced constipation by intragastric administration of probiotics Consti-Biome mixed with SynBalance SmilinGut (Lactobacillus plantarum PBS067, Lactobacillus rhamnosus LRH020, Bifidobacterium animalis subsp . The purified PCR products were amplified for final library construction using NexteraXT Indexed Primer. Mechanisms of action of probiotics. Fibre effects on intestinal functions (diarrhoea, constipation and irritable bowel syndrome). lactis MN-gup on constipation and the composition of gut microbiota. We are committed to providing the best education and services, and Natural Medicines of the highest quality and efficacy in order to help people live happier, healthier lives. Res. In G3, which had the highest increase among all groups, it increased by about 1.3 times compared to LOP. But why does this occur? The relationship between mRNA levels and proteins can be influenced by steady state, long-term state, and short-term adaptation. Fujian Academy of Agricultural Sciences, China. Clear (2016). Mucosal flora in Crohn's disease and ulcerative colitis-an overview. hbspt.forms.create({ SCFAs increased IL-18 production through a GPR109a-mediated pathway, which could sustain stomach homeostasis and prevent colorectal carcinogenesis (Kalina et al., 2002; Singh et al., 2014). Up to 38% of pregnant women experience constipation, which can be caused by prenatal supplements, hormonal fluctuations, or changes in physical activity (12). (D,E) Exhibit the alpha-diversity beta-diversity, respectively. Korean J. Med. Advertisement cookies are used to provide visitors with relevant ads and marketing campaigns. Acta 854, 8694. 2023 Healthline Media LLC. 305, C447C456. 10, 74167425. Improvement of loperamide-induced slow transit constipation by Bifidobacterium bifidum G9-1 is mediated by the correction of butyrate production and neurotransmitter profile due to improvement in dysbiosis. J. Gastroenterology 111, 16831699. Neurogastroenterol. Galacto-oligosaccharides supplementation in prefrail older and healthy adults increased faecal bifidobacteria, but did not impact immune function and oxidative stress. The Lactobacillus as a probiotic: focusing on liver diseases. PMID: 19608185. The Supplementary material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2023.1174968/full#supplementary-material. Microbiol. SEE PRODUCT 3. At the family level, Erysipelotrichaceae belonging to the phylum Firmicutes (now Bacillota) was found to increase in abundance in the G2 and G3 groups compared to the LOP (Figure 5B). Molecular defects in mucosal serotonin content and decreased serotonin reuptake transporter in ulcerative colitis and irritable bowel syndrome. Proc. Constipation is one of the most common gastrointestinal (GI) disorders worldwide. (2022). An increase in Akkermansia abundance was observed following alleviation of irritable bowel syndrome (IBS) and inflammatory bowel disease (IBD) (Png et al., 2010; Rajili-Stojanovi et al., 2013). Metagenics CandiBactin-BR - Concentrated Berberine Formula for Healthy Intestinal Support, Detoxification and Elimination Functions - 180 Count. doi: 10.1186/1472-6882-13-333, Kim, J. E., Park, J. W., Kang, M. J., Choi, H. J., Bae, S. J., Choi, Y. S., et al. It does not store any personal data. (2021). 12, 791801. Lubiprostone: a chloride channel activator for treatment of chronic constipation. doi: 10.1097/MED.0b013e32831e9c8e, Cani, P. D., and Knauf, C. (2021). UltraFlora BiomePro is a complex of eight clinically-studied probiotic strains to support gastrointestinal health and immune health.*. 200L of chloroform (Sigma Aldrich, St. Louis, MO, United States) was added to the sample, left at room temperature for 5min, and then centrifuged at 14,000rpm, 5min, 4C. doi: 10.1128/AEM.04050-14, Ros-Covin, D., Ruas-Madiedo, P., Margolles, A., Gueimonde, M., De Los Reyes-Gaviln, C. G., and Salazar, N. (2016). Microbes 12, 3142. 79, 147157. US Patent 11,026,897. Can. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, significantly different from the LOP as per one-way ANOVA with the Tukeys post-test. Butyrate showed an immunoregulatory effect and was reported to have therapeutic and protective effects against distal ulcerative colitis (Bcker et al., 2003; Wang et al., 2018). Learn how taking probiotics can benefit weight loss, heart health, digestion, immune function and more. Changes in indicators related to constipation by multi-strain probiotics Consti-Biome. Table 2. World J Gastroenterol: WJG 14, 62376243. *p<0.05, **p<0.01, significantly different from the LOP as per one-way ANOVA with the Dunnetts multi comparisons test. 80, 510523. doi: 10.1096/fj.14-259598, Reunanen, J., Kainulainen, V., Huuskonen, L., Ottman, N., Belzer, C., Huhtinen, H., et al. Soc. Rambaut, A. Microbiol. J. Physiol. doi: 10.1097/MIB.0b013e31827fec6d. Hansen), and Streptococcus thermophilus CKDB027 (Chong Kun Dang Bio) was obtained from Chong Kun Dang HealthCare (Seoul, Korea). When it comes to bowel regularity, your microbiome plays Biol. Park, M. I. Therefore, the multi-strain probiotics used in this experiment were thought to help alleviate LOP-induced constipation by altering the levels of short-chain fatty acids, serotonin, and mucin through improvement in the intestinal microflora. The anti-constipation effects of raffino-oligosaccharide on gut function in mice using neurotransmitter analyses, 16S rRNA sequencing and targeted screening. Microbiol. This can be reduced by constipation (Kim et al., 2019a) and restored by probiotics or substances or a combination of probiotics and other substances (Kim et al., 2013, 2019b; Huang et al., 2022; Tang et al., 2022). toxins that can harm your body, and, Fight pH of diluted the fecal samples in distilled water was measured by a Ohause Starter300 pH meter (Chen et al., 2019). These cookies track visitors across websites and collect information to provide customized ads. Two species of Akkermansia, namely, Akkermansia glycaniphila and Akkermansia muciniphila, were reported at the time of writing (www.bacterio.net/index.html; Parte, 2018). PLoS One 8:e76520. Evol. LP299v also seemed to regulate the expression of CD4+ lymphocytes, CD14+ monocytes, adipocytes, in addition to B cells.3. J. Gastroenterol. At the end of the multi-strain probiotics treatment, fecal samples were collected, then measured the number, pH, and water content, respectively. 20:946. doi: 10.3390/ijms20040946, Kim, J. E., Yun, W. B., Lee, M. L., Choi, J. Y., Park, J. J., Kim, H. R., et al. With its broad range of gut function specific influence, LP299v seems like a promising strain of Lactobacillus, and more research and clinical applications for this specific strain with relation to gut health and function are certainly warranted. Inflamm. J. Korean Soc. In animal studies, A. muciniphila and its extracellular vesicles alleviated colitis induced by dextran sulfate sodium (Kang et al., 2013; Zhai et al., 2019). What impact is it having within your gut? * . Lactobacillus spp. Treatment of constipation. All specimens were evaluated by the same pathologist (SHH), who was masked as to whether the specimens came from comparative or control groups. (2021). Numerous studies have shown that the microbiome affects host health through various mechanisms. doi: 10.1016/S0016-5085(96)70034-3, Costedio, M. M., Hyman, N., and Mawe, G. M. (2007). Metagenics Institute supports a diverse practitioner base to optimize patient outcomes by shifting existing paradigms in healthcare. 116, 11731182. doi: 10.1038/ajg.2010.281, Rajili-Stojanovi, M., Shanahan, F., Guarner, F., and De Vos, W. M. (2013). Butyrate belongs to the SCFA family of volatile fatty acids, with 16 carbon atoms attached to the aliphatic chain (Ros-Covin et al., 2016). Butyrate can maintain barrier integrity by accelerating GI transit (Soret et al., 2010; Zhuang et al., 2019). For untargeted metabolites analysis, the cecal contents prepared above were chromatographically separated by Ultmate-3,000 UPLC system (Thermo Fisher Scientific,Waltham, MA, United States) equipped with 100mm2.1mm UPLC BEH 1.7m C18 column (Waters, Milford, MA, United States) and 5.0mm2.1mm UPLC BEH 1.7m C18 VanGuard Pre-Column (Waters, Milford, MA, United States). Bifidobacteria can protect from enteropathogenic infection through production of acetate. 17, 655659. The Akkermansia genus belongs to the phylum Verrucomicrobiota. (2011). Tnf-, which is known to be increased in inflammatory diseases, showed a tendency to increase in all constipation-induced groups. Int. Constipation is one of the most common gastrointestinal (GI) disorders worldwide. We believe in personalised, holistic medicine that addresses all drivers of dysfunction and disease in order to understand the underlying cause and achieve optimal health. [4] Ringel-Kulka T, Choi CH, Temas D, Kim A, Maier DM, Scott K, et al. Here, the mixture of the SmilinGut and other probiotics showed an improvement effect on symptoms related to constipation. These cookies will be stored in your browser only with your consent. Chronic constipation: a review of literature. Description Reviews (2) Label & Supplement Facts Ingredients: Microcrystalline cellulose, capsule (hydroxypropylmethylcellulose), Lactobacillus acidophilus NCFM, magnesium stearate (vegetable), Bifidobacterium lactis Bi-07, and silica. The genus Akkermansia, which was increased in this experiment (Figure 5C), was recently proposed as a next-generation probiotic (Liu Y. et al., 2022). Int. Potential of Lactobacillus plantarum ZDY2013 and Bifidobacterium bifidum WBIN03 in relieving colitis by gut microbiota, immune, and anti-oxidative stress. For principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) analysis of metabolites, SIMCA 17 (Umetrics AB, Umea, Sweden) was used. Anything less than this, and you might be experiencing constipation. Valerate is also known to correlate with other SCFAs and the microbiome (Lu et al., 2022). Eating fermented foods with live probiotics can have powerful benefits for your body and brain. Opin. doi: 10.1172/JCI120901, Singh, N., Gurav, A., Sivaprakasam, S., Brady, E., Padia, R., Shi, H., et al. The use of probiotics to improve constipation is well known. 66, 112. Data are meanSEM of 7 animals. doi: 10.1038/nature09646, Fukumoto, S., Tatewaki, M., Yamada, T., Fujimiya, M., Mantyh, C., Voss, M., et al. Enterochromaffin cells use tryptophan hydroxylase (TPH) to synthesize 5-HT. See additional information. Protein from the colonic tissue was quantified by the bicinchoninic assay. doi: 10.1016/S0140-6736(72)92974-1, Caballero-Franco, C., Keller, K., De Simone, C., and Chadee, K. (2007). Nutrients 13:2238. doi: 10.3390/nu13072238, Zhao, Y., Liu, Q., Hou, Y., and Zhao, Y. Gut motility was increased after Lactobacillus plantarum was administered to subjects with constipation (Kusumo et al., 2019). In addition, the number, pH, and water content in stools were improved. doi: 10.1111/j.1365-2982.2011.01819.x, Jeong, J.-J., Park, H. J., Cha, M. G., Park, E., Won, S.-M., Ganesan, R., et al. (2010). (2011). 33, 10731075. Survival of Lactobacillus plantarum DSM 9843 (299v), and effect on the short-chain fatty acid content of faeces after ingestion of a rose-hip drink with fermented oats. doi: 10.1053/j.gastro.2010.01.053. Ducrott P, Sawant P, Jayanthi V. Clinical trial: Innovative Structured Nutrition Therapy for Compromised Gut (IBS and IBD), Antibiotics: Effects & How to Restore Healthy Gut Bacteria, Probiotics: What They Are and How to Use Them Effectively. Serotonin (5-HT) levels (ng/ml) in colon samples. Here is a list of 11 super healthy probiotic foods. In addition, they are also involved in colonic serotonin production (Reigstad et al., 2015). This creates more good bacteria that then produce more SCFAs, which we now know is very useful for combatting constipation! As Molecules 27:2235. doi: 10.3390/molecules27072235, Liu, M.-J., Yang, J.-Y., Yan, Z.-H., Hu, S., Li, J.-Q., Xu, Z.-X., et al. (B); number of fecal pellets, (C); Fecal pH, (D); Fecal water content, (E); gastrointestinal transit rate of normal control (NOR), loperamide administration (LOP), positive control (PC), low concentration multi-strain probiotics administration (G1, 2108cfu/mL), medium concentration multi-strain probiotics administration (G2, 2109cfu/mL), and high concentration probiotic administration (G3, 21010cfu/mL) groups. In a 4-week study in 60 pregnant women with constipation, eating 10.5 ounces (300 grams) of probiotic yogurt enriched with Bifidobacterium and Lactobacillus bacteria daily increased the frequency of bowel movements and improved several constipation symptoms (13). doi: 10.3748/wjg.14.6237, Ye, Y., Wang, Y., Yang, Y., and Tao, L. (2020). Acetate is involved in cholesterol synthesis and shows a protective effect against infection by Escherichia coli O157:H7 (Arora and Sharma, 2011; Fukuda et al., 2011). entire body healthy. The abundances of the phylum Verrucomicrobia, the family Erysipelotrichaceae and the genus Akkermansia were increased in fecal samples of the probiotic-treated groups. Around 16% of people undergoing this cancer treatment experience constipation (17). Propionate inhibits cholesterol synthesis (Henningsson et al., 2001), and valerate has a positive effect on the pathogenesis of colitis (Yuille et al., 2018). Data are meanSEM of 5 animals in (B). Induction of constipation with loperamide is known to decrease the thickness of the intestinal mucosa and to delay the movement of contents in the intestine (Sin et al., 2010). Our mission is to transform healthcare by inspiring and educating practitioners, and their patients, about personalized lifestyle medicine. In brief, each fecal sample was mixed with (acetonitrile:3-dilstilled water, 1:1, volume:volume) and then homogenized with a Mixer Mill MM400 (Haan, Germany, Letsch GmbH & Co.). change in stool appearance The exact cause of IBS remains uncertain. Probiotics are effective in improving various diseases, such as GI diseases, allergies, respiratory diseases, neurological and psychiatric diseases, genitourinary tract infections, and oral diseases (Jeong et al., 2022). Food Drug Anal. Nat. For pathological analysis of the intestine, intestinal specimens were fixed with 10% formalin, routinely embedded in paraffin; the tissue sections were processed with hematoxylin and eosin. J. Ethnopharmacol. To read more on what probiotics are and why selecting the right strain is important, click here. Storage: Keep refrigerated. Lactobacillus plantarum 299v (LP299v) has demonstrated regulatory action on genes modulating various activities, including inflammation in pig intestine. The serotonin level of PC was 2.7ng/mL, showing an increasing trend compared with the LOP group, but there was no statistically significant difference (Figure 3). These mixed samples were kept at 40C for 30min and diluted with 70% ACN to 2mL (Han et al., 2015). Nutr. Microbiome of normal control (NOR), loperamide administration (LOP), positive control (PC), low concentration multi-strain probiotics administration (G1, 2108cfu/mL), medium concentration multi-strain probiotics administration (G2, 2109cfu/mL), and high concentration probiotic administration (G3, 21010cfu/mL) groups were analyzed with fecal samples 2weeks after treatment with multi-strain probiotics. Commun. 23, 107113. Reduced frequency of defecation (less than three bowel motions per week); A sensation of not passing the entire stool (i.e. Probiotics are often used to aid IBS symptoms, including constipation. Ambizas, E. M., and Ginzburg, R. (2007). Adhesive Bifidobacterium induced changes in cecal microbiome alleviated constipation in mice. symptoms are benign or harmless. doi: 10.1016/j.jfda.2019.06.005, Coates, M. D., Mahoney, C. R., Linden, D. R., Sampson, J. E., Chen, J., Blaszyk, H., et al. [11] Ducrott P, Sawant P, Jayanthi V. Clinical trial: Lactobacillus plantarum 299v (DSM 9843) improves symptoms of irritable bowel syndrome. Br. For PCR, 10pg. 20, 314. LPSNlist of prokaryotic names with standing in nomenclature (Bacterio. (2022). A total of 256 valid metabolites from all samples were identified through LCMS analysis. Endocrinol. under this definition, straining to pass a hard stool each day, or only going Cutadapt removes adapter sequences from high-throughput sequencing reads. PCoA visualized the relationship between samples (Rambaut, 2009; Caporaso et al., 2010). Microbiome changes by induction of constipation and intake of multi-strain probiotics Consti-Biome. Talk to a Natural Healthcare Practitioner about accessing this constipation-combatting duo today! Extracellular vesicles derived from gut microbiota, especially Akkermansia muciniphila, protect the progression of dextran sulfate sodium-induced colitis. Medicine 97:e10631. They have a variety of mechanisms, such as competition with pathogens, secretion of bacteriocins, fatty acid production, enzymatic activities, and microbiome changes (Plaza-Diaz et al., 2019).
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