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Iodoform test: Methyl ketones are oxidized by sodium hypoiodite to give yellow ppt. Google Scholar. Publishers note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Ethyl benzoate (lane #4) was unreactive to both. Ramadan, K., Shevelev, I. V., Maga, G. & Hbscher, U. Res. Viewing a TLC plate under ultraviolet light is non-destructive, while using a chemical stain is destructive. Interfaces 3, 37623771 (2011). Chem. Compared to other stains, this stain has a somewhat short shelf life (approximately half a year). Terms of Use and Privacy Policy: Legal. 126, 59315936 (2014). Chem. 5B). As such, both proteins and nucleic acids, as well as a wide variety of organic and inorganic polymeric materials, may be subjected to a successful AP/BP-mediated cross-linking. Both acetophenone and benzophenone are organic compounds that fall under the category of ketones because both these compounds have a carbonyl carbon with alkyl or aryl groups attached to both sides. It did not stain with permanganate after elution even though the compound is reactive to the stain (an undiluted, uneluted sample of 2-pentene did stain somewhat on a scrap TLC plate, Figure 2.45d). Chem. The key difference between acetophenone and benzophenone is that acetophenone has a methyl group and a benzene ring attached to the carbonyl carbon, whereas benzophenone has a benzene ring attached to the carbonyl carbon. Safety note: wear gloves while using this highly acidic stain. Mater. Then the samples were transferred from parafilm wells to microtubes and quenched with 2loading solution. Our strategy for the synthesis of reactive ON photoprobes bearing AP- or BP-functional groups exploited the TdT-based template-independent 3-elongation. RSC Adv. 11, 51625172 (2013). Biotechnol. We find that terminal deoxynucleotidyl transferase-mediated 3-tailing using AP/BP-containing modified nucleotides generates photoactive DNA, suitable for a straightforward covalent cross-linking with both interacting proteins and a variety of well-known solid polymeric supports. A sheet of parafilm was placed over the top of a 96-well plate, and taped to the plate on all four sides. J. TdT, T4 polynucleotide kinase (T4 PNK), solutions of 2-deoxyribonucleoside triphosphates, Zeba Spin desalting columns and BS glass slides were purchased from Thermo Fisher Scientific Baltics (Vilnius, Lithuania). Legal. Chem Comm. The additional nonpolar bulk of benzophenone makes it dissolve better in the weakly polar eluent, causing it to spend more time in the mobile phase. The yellow-green PMA reagent \(\left( \ce{Mo^{6+}} \right)\) oxidizes the compound on the plate while itself being reduced to molybdenum blue (\(\ce{Mo^{5+}}\) or \(\ce{Mo^{4+}}\)). (A) A scheme of the photo-cross-linking between 3-modified DNA and DNA-binding protein upon exposure to UV365 nm light. This provided dose of UV irradiation of ~8J/cm2. & He, C. A diazirine-based nucleoside analogue for efficient DNA interstrand photocross-linking. declare potential financial interests in the future development and commercialization of the N4-acylcytidines. Summary. Horkov, P. et al. Simple and sensitive microRNA labeling by terminal deoxynucleotidyl transferase. Google Scholar. Immediately after irradiation, the specimens were rinsed with 500L of wash buffer (50mM potassium phosphate, 1% Triton X-100 (pH 7.0)) following incubation (1h at room temperature) in 1.5mL of wash buffer vigorously shaking. This stain has a high shelf life (5+ years). Ming, X. The relative percentage of immobilization is indicated at the bottom where the most efficient immobilization was chosen to serve as 100%. We can also convert it into styrene, and it is useful for the production of many pharmaceuticals as well. In general, the yield of the cross-linking strongly depended on the number of photoreactive groups present on a DNA molecule. Table 2.4: Summary table for TLC visualization methods. We find that . Bioorg. Biophys. (C) A schematic representation of AP- or BP-induced DNA photoimmobilization. 87, 8189 (2015). The site owner may have set restrictions that prevent you from accessing the site. Sci. Sakellari, I. et al. The antifungal activity of three schiff bases (vanillin thiosemicarbazone, benzophenone thiosemicarbazone and acetophenone thiosemicarbazone) and two schiff base complexes vanillin . We are not permitting internet traffic to Byjus website from countries within European Union at this time. Qiu, Z., Lu, L., Jian, X. This is an example of when the staining results "make structural sense", and can even support the identification of unknown spots. In summary, the key difference between acetophenone and benzophenone is that the acetophenone has a methyl group and a benzene ring attached to the carbonyl carbon, whereas benzophenone has a benzene ring attached to the carbonyl carbon. Consequently, complicated surface preparation techniques affect the experimental design and limit the choice of relatively inert polymeric supports. To verify that the attached 3-dCBP-tailed DNA probes are properly oriented for a successful detection of complementary DNA, we performed a targetprobe hybridization procedure. DNA immobilization and hybridization play a major role in the era of nanostructures, therefore all challenges and drawbacks of various immobilization procedures need to be acknowledged. The compound in lane #4 of all the plates (ethyl benzoate) was unreactive to anisaldehyde stain, but could be visualized with UV and \(\ce{I_2}\). Article 3. (B) Autoradiograms of SDS-PAGE showing covalent bioconjugates of the indicated 3-modified ONs and TdT. 53, 1458014582 (2014). The specific stain should be chosen based on the presumed structure of the compounds you want to visualize. Many spots will appear yellow-brown almost immediately, and may darken with extended time. Am. Proteome Sci. Gently heat the plate to develop the spots. (iii) Phenol and benzoic acid can be distinguished by ferric chloride test. The key difference between acetophenone and benzophenone is that acetophenone has a methyl group and a benzene ring attached to the carbonyl carbon, whereas benzophenone has a benzene ring attached to the carbonyl carbon. Photolabeling of calmodulin with a synthetic calmodulin-binding peptide. Photoclick postsynthetic modification of DNA. Our results establish novel N4-cytosine nucleobase modifications as photoreactive labels and suggest an effortless approach for photoimmobilization of nucleic acids. Dankbar, D. M. & Gauglitz, G. A study on photolinkers used for biomolecule attachment to polymer surfaces. Kabouraki, E. et al. and JavaScript. Flett, F. J., Walton, J. G., Mackay, C. L. & Interthal, H. Click chemistry generated model DNA-peptide heteroconjugates as tools for mass spectrometry. Biol. The used nucleotides are indicated above the lanes. Note that in Figure 2.21c all spots maintained their relative order but traveled to a greater height on the plate and increased their \(R_f\) values (Table 2.2) in the more polar eluent. Correspondence to Synthetic ONs were purchased from Metabion (Planegg/Steinkirchen, Germany); sequences of the ONs are shown in Table1. Acta Biochim. Hard. Prog. Ed. Curr. There are a variety of destructive visualization methods that can turn colorless compounds on a TLC plate into colored spots. Lett. Silica gel (shown in Figure 2.16) is composed of a network of silicon-oxygen bonds, with \(\ce{O-H}\) bonds on its surface, as well as a layer of water molecules. The bromocresol green stain is specific for acidic compounds, and should be able to visualize compounds that produce a solution lower than pH 5. Acetophenone is an organic compound having the chemical formula C8H8O while Benzophenone is an organic compound having the chemical formula C13H10O. The p-anisaldehyde and vanillin stains react in a similar manner, and commonly undergo Aldol and acetalization reactions to produce highly conjugated (and thus colored) compounds on TLC plates. Moreover, the addition of an excessive amount of TdT led to an even more effective formation of covalent complexes. Roychoudhury, R., Jay, E. & Wu, R. Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase. The Journal of Physical Chemistry A 2005, 109 (27) , 5989-5994. Moderately polar compounds have a greater attraction to the mobile phase. Adv. Chembiochem 12, 18541857 (2011). When considering the uses of benzophenone, it is useful as a building block for the synthesis of many organic compounds, as a photo-initiator in UV curing applications, as a UV blocker for plastic packages, etc. The physical and analytical data such as physical constant viz. Andrade, P., Martn, M. J., Jurez, R., de Saro, F. L. & Blanco, L. Limited terminal transferase in human DNA polymerase defines the required balance between accuracy and efficiency in NHEJ. Shigdel, U. K., Zhang, J. Further, a successful photoimmobilization of AP- or BP-modified DNA was accomplished using PP, PLA, PDMS, BS as substrates as well as metal-containing (Al, Ti, Zr or V) sol-gel based hybrid materials (Fig. Two-photon polymerization of titanium-containing sol-gel composites for three-dimensional structure fabrication. If the length of the modified DNA must be constant, or the amount of the targeted protein is limiting, one can prolong the time of irradiation rather than vary the critical parameters. This mixed solvent is mostly nonpolar due to the high percentage of hexane, but is more polar than straight hexane, due to the presence of some ethyl acetate (which has polar bonds, Figure 2.21a). The fluorination of benzophenone in anhydrous HF furnished predominantly m -fluorobenzophenone, whereas in . It should be discarded if it acquires a blue color. Then the specimens were rinsed with 200L of distilled water and air-dried. Since the compounds remain unchanged after viewing with UV light, a further visualization technique can be used afterwards on the same plate. Raindlov, V., Pohl, R. & Hocek, M. Synthesis of aldehyde-linked nucleotides and DNA and their bioconjugations with lysine and peptides through reductive amination. Hybrid material films containing aluminium, titanium, zirconium or vanadium were prepared on BS slides as described previously68,69,70. 43, e110, https://doi.org/10.1093/nar/gkv544 (2015). 2. Dormn, G., Nakamura, H., Pulsipher, A. As eluent flows over the sample (Figure 2.16b), an equilibrium is established between the sample being adsorbed on the stationary phase and dissolved in the mobile phase. CAS Considering that TdT is known to extend ss- or ds- DNA58,59, RNA substrates60 as well as to perform de novo synthesis of polynucleotides61, the proposed method is of paramount importance since it can be easily adopted for the cross-linking of a wide spectrum of nucleic acids. { "2.3A:_Overview_of_TLC" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.3B:_Uses_of_TLC" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.3C:_The_Retention_Factor" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.3D:_Separation_Theory" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.3E:_Step-by-Step_Procedures_for_Thin_Layer_Chromatography" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.3F:_Visualizing_TLC_Plates" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, { "2.01:_Prelude_to_Chromatography" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.02:_Chromatography_Generalities" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.03:_Thin_Layer_Chromatography_(TLC)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.04:_Column_Chromatography" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.05:_Gas_Chromatography_(GC)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, [ "article:topic", "vanillin", "authorname:nicholsl", "TLC Plates", "Permanganate", "iodine", "p-Anisaldehyde", "Phosphomolybdic Acid (PMA)", "Iron(III) Chloride", "Bromocresol Green", "Visualizing TLC", "showtoc:no", "license:ccbyncnd", "licenseversion:40", "source@https://organiclabtechniques.weebly.com/" ], https://chem.libretexts.org/@app/auth/3/login?returnto=https%3A%2F%2Fchem.libretexts.org%2FBookshelves%2FOrganic_Chemistry%2FOrganic_Chemistry_Lab_Techniques_(Nichols)%2F02%253A_Chromatography%2F2.03%253A_Thin_Layer_Chromatography_(TLC)%2F2.3F%253A_Visualizing_TLC_Plates, \( \newcommand{\vecs}[1]{\overset { \scriptstyle \rightharpoonup} {\mathbf{#1}}}\) \( \newcommand{\vecd}[1]{\overset{-\!-\!\rightharpoonup}{\vphantom{a}\smash{#1}}} \)\(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\) \(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\)\(\newcommand{\AA}{\unicode[.8,0]{x212B}}\), 2.3E: Step-by-Step Procedures for Thin Layer Chromatography. 16, 1931 (2017). The results may prove useful for both the development of a feasible and direct DNA photoimmobilization approach as well as for the discovery of novel DNA-protein interactions. PubMed Central Chem. & Pomerantz, R. T. Polymerase is a robust terminal transferase that oscillates between three different mechanisms during end-joining. The ease and efficiency of DNA end functionalization using automated solid-phase synthesis dictates the choice of modification14. 328, 6372 (2003). Rev. The reaction mixtures were incubated for 5min at 37C. Vigorous heating is required to develop the spots, but the plate is overheated when the background begins to darken. Figure 2.16 shows how acetophenone would cling to the surface of silica gel through intermolecular forces (IMF's). The "high" setting can be used at first, with the setting turned to "low" if the plate begins to char. Google Scholar. And, this compound occurs as a colourless, viscous liquid. The rate for the reaction of O- with . https://doi.org/10.1093/nar/gky435 (2018). Side by Side Comparison Acetophenone vs Benzophenone in Tabular Form Benzophenone was crystallized, dissolved in anhydrous THF and treated with NaBH4 under nitrogen, at room temperature for 48 hrs10. Thermal denaturation, the most frequent and simple method of DNA denaturation, further ensures compatibility of the immobilized N4-acyl-dC-DNA probes for DNA hybridization based assays. Figure2 shows the formation of covalent conjugates after exposure to a 365nm UV light, where DNA-TdT cross-links are represented by the bands with lower mobility. Both compounds are able to hydrogen bond to the polar stationary phase (Figure 2.19a+b), so are more strongly attracted to the stationary phase than ethylbenzene, which interacts only through weak London dispersion forces (Figure 2.19c). Hollenstein, M. Deoxynucleoside triphosphates bearing histamine, carboxylic acid, and hydroxyl residues synthesis and biochemical characterization. Then the specimens were placed under the UV light source (5mm away from the surface of the light source) and irradiated for 5min (3655nm). Next, we performed a covalent attachment of ssDNA, via its modified 3-mAP- or mBP-containing tail, to a variety of intact solid substrates. Acetophenone being a methyl ketone responds to this test, but benzophenone does not. 45, 25832602 (2016). If the compound has a low boiling point, it probably evaporated during elution. Med. When considering the uses of acetophenone, in commercial scale, it is important as a precursor for the production of resins, as an ingredient in fragrances, etc. Since both para-isomers were revealed to be better substrates for TdT than ortho- or meta-analogues53, the cross-linking using pAP- or pBP-containing DNA was the most efficient (Fig. 261, 1069510700 (1986). The reagent is expensive, but the stain has a very long shelf life (5+ years). J. Mol. The apparatus consisted of an ice container, a 96-well plate, a sheet of parafilm and an UV light source. 22, 924932 (2006). For example, a TLC of benzaldehyde visualized with UV light (Figure 2.48a) shows two spots, and based on relative \(R_f\) values, it would make sense that the dark spot is benzaldehyde and the fainter spot near the baseline is benzoic acid (caused by an oxidation of benzaldehyde). This stain is susceptible to degradation by light, so store wrapped in aluminum foil (Figure 2.37e), ideally in the refrigerator when not in use. Sontheimer, E. J. Site-specific RNA crosslinking with 4-thiouridine. Because acetophenone is a methyl ketone, it responds to this test, but not benzophenone. Recipe: \(5 \: \text{g}\) phosphomolybdic acid in \(500 \: \text{mL}\) ethanol. Synthesis of site-specific DNA-protein conjugates and their effects on DNA replication. Aliphatic hydrogens or hydrogens adjacent to hydroxyl groups can serve as H-donors, depending on H steric accessibility and close proximity to AP/BP. 53, 1303113034 (2017). Shrinking hydrogel-DNA spots generates 3D microdots arrays. Soc. Gently shake the chamber to bury the TLC plate in the iodine-stained silica or alumina (Figure 2.33c) until the spots become colored (Figure 2.33d). We can abbreviate it as Ph2O Ph refers to phenol (another name for benzene ring). The actual structure of these complexes is debated\(^6\). Solgel based materials for biomedical applications. PubChem Compound Database, U.S. National Library of Medicine, Available here. The key difference between acetophenone and benzophenone is that acetophenone has a methyl group and a benzene ring attached to the carbonyl carbon, whereas benzophenone has a benzene ring attached to the carbonyl carbon. (Note: alcohols, carboxylic acids, and alkyl halides often do not stain.). Dhende, V. P., Samanta, S., Jones, D. M., Hardin, I. R. & Locklin, J. One-step photochemical synthesis of permanent, nonleaching, ultrathin antimicrobial coatings for textiles and plastics. It is therefore important to be cautious in using TLC to interpret the quantity of material present in a sample, for example when assessing the quantity of an impurity (such as in lane #3 of Figure 2.46, which contains cinnamaldehyde and its impurity cinnamic acid). Recipe (Vanillin): \(250 \: \text{mL}\) ethanol, \(15 \: \text{g}\) vanillin, and \(2.5 \: \text{mL}\) concentrated \(\ce{H_2SO_4}\). Acetophenone (C 6 H 5 COCH 3) contains the group CH 3 CO attached to carbon and hence given iodoform test while benzophenone does not contain this group and hence does not give iodoform test.. C 6 H 5 COCH 3 (Acetophenone) + 3I 2 + 4 NaOH CHI 3 + C 6 H 5 COONa (Iodoform) + 3 NaI + 3 H 2 O . Photoimmobilization of AP- or BP-containing DNA to a PS support. Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekio al. analysed the data; R.M., J.J. and D.T. Further visualization may be attempted after the color fades. Chem. The impurity present on the baseline of lane #3 (the impurity cinnamic acid) was strongly UV active, but could hardly be seen with the other stains. This work was supported by the European Unions Horizon 2020 research and innovation program [BlueGrowth: Unlocking the potential of Seas and Oceans] under grant agreement no. Angew. Most other functional groups do not absorb UV light at the wavelengths used and will not appear dark under the UV lamp even though they are still there. 52, 1051510518 (2013). If the compound is expected to be UV active, check the plate under UV light, and if necessary spot more times before elution. The used hybridization and denaturation conditions are indicated. This stain is light sensitive and should be stored wrapped in aluminum foil in the refrigerator. wrote the paper. In almost all applications of TLC, the stationary phase is a silica or alumina adsorbent and the mobile phase is an organic solvent or solvent mixture (the "eluent") that rises up the plate (equation 3). 1, 161176 (2015). Under the acidic conditions of the stain, some aldehydes or ketones can undergo a keto-enol tautomerism, and the enol can undergo acid-catalyzed nucleophilic addition to p-anisaldehyde or vanillin through an aldol mechanism. & Shionoya, M. Enzymatic synthesis of ligand-bearing DNAs for metal-mediated base pairing utilising a template-independent polymerase. Aryl ketones, aryl azides and diazirines are the most popular photoreactive groups used for the photo cross-linking30,31. We would also like to show our gratitude to Dr Laura Kaliniene for assistance with pulsed-field electrophoresis and critical reading of the manuscript. If a dark spot is seen with a UV lamp, it is customary to circle the spot with pencil (as in Figure 2.46b), as the spot will be invisible when the lamp is removed. Martin, T. A. et al. -- Tom Jupille. Autoradiograms of UVA-induced photoimmobilization of dCmAP-ONs and dCmBP-ONs to different solid supports. (A) A scheme visualizing hybridization experiment. A sequential pathway for acetophenone and benzophenone electrocatalytic hydrogenation via their corresponding alcohols as intermediate species was established from different experimental approaches: OCP potentiometry and bulk electrolysis. Besides, we can get it as a byproduct from the oxidation of ethylbenzene to form ethylbenzene hydroperoxide. The relative order of \(R_f\) reflects the polarity trend in the series. For example, the plate in Figure 2.47 was visualized with p-anisaldehyde stain, and Figure 2.47a shows how the plate appeared immediately after heating. (B) An autoradiogram of hybridization of 5-radiolabelled ssDNA targets to photoimmobilized BP-bearing complementary DNA probes. 3 indicate that a BP-functionalization induced a more effective immobilization. For example, the compound in lane 2 of Figure 2.46 (acetophenone) can be easily seen with ultraviolet light (Figure 2.46a), but on the plate visualized with iodine (Figure 2.46c), the pencil markings encapsulate a larger region than is seen darkened by the iodine. It reacts with NaHCO 3 to form a sodium salt alongwith evolution of CO 2. Hybridization and denaturation results were then visualized by phosphorimaging. Overview and Key Difference As such, oligonucleotide (ON) bioconjugates hold promise for targeted drug delivery3,4, bioimaging5,6, biosensing7,8, as well as for the development of DNA nanomaterials9,10 and DNA-based nanodevices11,12. Considering its properties, the molar mass is 120.15 g/mol. A solution to this problem is to run the TLC again and visualize it immediately. To investigate the dependence of the photoimmobilization efficiency on the number of photoreactive groups present in a nucleic acid molecule, the duration of the 3-tailing reaction was varied. CAS Furthermore, the molar mass of acetophenone is 120.15 g/mol, while the molar mass of benzophenone is 182.22 g/mol. Give a simple chemical test to distinguish between the following pair of compounds:Acetophenone and Benzophenone. Overall, our results indicated that BP-containing DNA may become a promising alternative for a delicate and low-cost photoimmobilization, hybridization as well as denaturation. Biosci. Google Scholar. It is likely that a large spot is present in a greater quantity than a small spot, but it could also be that the large spot is more responsive to the visualization technique. Langmuir 25, 1395213958 (2009). Functional DNA-containing nanomaterials: cellular applications in biosensing, imaging, and targeted therapy. Recipe: \(1.5 \: \text{g}\) \(\ce{KMnO_4}\), \(10 \: \text{g} \: \ce{K_2CO_3}\), \(1.25 \: \text{mL} \: 10\% \: \ce{NaOH} \left( aq \right)\), and \(200 \: \text{mL}\) water. Biol. Chem. An "iodine chamber" can be created by adding a few iodine crystals to a TLC chamber, or by adding a few iodine crystals to a chamber containing a portion of powdered silica or alumina (Figure 2.33a). To do this, deliver a small spot of sample on the baseline, and let it fully dry (it helps to blow on it) before delivering another spot over top of the first. If UV, iodine, or a stain fails to visualize a compound, it could mean the compound is simply not reactive to the technique, and another method should be tried. Kent, T., Mateos-Gomez, P. A., Sfeir, A. By submitting a comment you agree to abide by our Terms and Community Guidelines. The images or other third party material in this article are included in the articles Creative Commons license, unless indicated otherwise in a credit line to the material.

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